Hotspot

accD-psaI

LSC · 54973-55706 · 734 bp

Back to Cladopus

Pi 0.0123

MarkerSeek score 68.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 17
pi: 0.0123
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTTCACAAGAAGCGGAAATG	GTCATTGCAATTGCCGGAAA	47.6	45.0	58.1	57.9	912-928 bp	1.0	73.0
accD-psaI_p2	GGTTCACAAGAAGCGGAAATG	GAAGTCATTGCAATTGCCGG	47.6	50.0	58.1	58.4	915-931 bp	1.0	70.7
accD-psaI_p3	GGTTCACAAGAAGCGGAAAT	GTCATTGCAATTGCCGGAAA	45.0	45.0	56.6	57.9	912-928 bp	1.0	70.6
accD-psaI_p4	GGTTCACAAGAAGCGGAAATG	AGAAGTCATTGCAATTGCCG	47.6	45.0	58.1	57.4	916-932 bp	1.0	68.5
accD-psaI_p5	GGTTCACAAGAAGCGGAAAT	GAAGTCATTGCAATTGCCGG	45.0	50.0	56.6	58.4	915-931 bp	1.0	68.2