Hotspot

petN-trnC-GCA

LSC · 27423-28289 · 867 bp

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Pi 0.0186

MarkerSeek score 63.90

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 30
pi: 0.0186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	GGGGGACTGCAAATCCTTTT	50.0	50.0	58.2	58.4	932-946 bp	1.0	79.6
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	907-921 bp	1.0	74.1
petN-trnC-GCA_p3	GCCCAAGCGAGACTTACTAT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.1	58.4	931-945 bp	1.0	73.7
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	909-923 bp	1.0	73.7
petN-trnC-GCA_p5	ATTAAAGCAGCCCAAGCGAG	GGGGGACTGCAAATCCTTTT	50.0	50.0	58.9	58.4	940-954 bp	1.0	73.6