Hotspot

trnT-GGU-trnE-UUC

LSC · 24933-25577 · 645 bp

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Pi 0.0183

MarkerSeek score 57.00

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 23
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	CGTGAAAGGGAGATGTCCTG	50.0	55.0	58.0	58.0	598-740 bp	1.0	86.4
trnT-GGU-trnE-UUC_p2	CCATGGCGTTACTCTACCAC	CGTGAAAGGGAGATGTCCTG	55.0	55.0	58.1	58.0	577-719 bp	1.0	86.1
trnT-GGU-trnE-UUC_p3	TGGCGTTACTCTACCACTGA	CGTGAAAGGGAGATGTCCTG	50.0	55.0	58.1	58.0	574-716 bp	1.0	85.9
trnT-GGU-trnE-UUC_p4	GAACCGATGACTTACGCCTT	AGATGTCCTGAACCGCTAGA	50.0	50.0	58.0	57.9	588-730 bp	1.0	85.4
trnT-GGU-trnE-UUC_p5	CCGATGACTTACGCCTTACC	CGTGAAAGGGAGATGTCCTG	55.0	55.0	58.2	58.0	595-737 bp	1.0	85.3