Hotspot

trnG-GCC-psbZ

LSC · 20159-20631 · 473 bp

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Pi 0.019

MarkerSeek score 65.30

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 17
pi: 0.019
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGGATAGCGGGAATCGAAC	TTCTTCTCCTGATGGGTGGT	57.9	50.0	58.2	58.0	627-651 bp	1.0	75.2
trnG-GCC-psbZ_p2	GATAGCGGGAATCGAACCC	TTCTTCTCCTGATGGGTGGT	57.9	50.0	57.8	58.0	625-649 bp	1.0	75.0
trnG-GCC-psbZ_p3	GGATAGCGGGAATCGAACC	TTCTTCTCCTGATGGGTGGT	57.9	50.0	57.8	58.0	626-650 bp	1.0	75.0
trnG-GCC-psbZ_p4	CGGATAGCGGGAATCGAAC	TTCTCCTGATGGGTGGTCAA	57.9	50.0	58.2	58.3	624-648 bp	1.0	73.5
trnG-GCC-psbZ_p5	GATAGCGGGAATCGAACCC	TTCTCCTGATGGGTGGTCAA	57.9	50.0	57.8	58.3	622-646 bp	1.0	73.4