Hotspot

rps12

IRb · 102677-103481 · 805 bp

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Pi 0.00248

MarkerSeek score 60.20

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: IRb
length_bp: 805
variable_sites: 2
pi: 0.00248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	TGACATGAGCGTGAAAGAGG	ATTGAACGAGGAGCCGTATG	50.0	50.0	57.9	57.8	954 bp	1.0	77.7
rps12_p2	GACATGAGCGTGAAAGAGGT	ATTGAACGAGGAGCCGTATG	50.0	50.0	57.9	57.8	953 bp	1.0	77.7
rps12_p3	CAGTACCTCGACGTGACATG	ATTGAACGAGGAGCCGTATG	55.0	50.0	58.1	57.8	967 bp	1.0	77.5
rps12_p4	TGACATGAGCGTGAAAGAGG	AAGAATTGAACGAGGAGCCG	50.0	50.0	57.9	58.3	958 bp	1.0	77.3
rps12_p5	GACATGAGCGTGAAAGAGGT	AAGAATTGAACGAGGAGCCG	50.0	50.0	57.9	58.3	957 bp	1.0	77.3