Hotspot

rpoC2

LSC · 17118-21251 · 4134 bp

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Pi 0

MarkerSeek score 35.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4134
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TCCATAATCGGACCAGAGAGT	CCCGATCCATGCAGTAACAT	47.6	50.0	57.7	57.7	1104 bp	1.0	67.4
rpoC2_p2	TCCATAATCGGACCAGAGAGT	ATCCCGATCCATGCAGTAAC	47.6	50.0	57.7	57.4	1106 bp	1.0	65.1
rpoC2_p3	ACGAAATCCATAATCGGACCA	CCCGATCCATGCAGTAACAT	42.9	50.0	57.2	57.7	1110 bp	1.0	63.3
rpoC2_p4	TCCATAATCGGACCAGAGAGT	TCCCGATCCATGCAGTAACA	47.6	50.0	57.7	58.8	1105 bp	1.0	63.1
rpoC2_p5	CCATAATCGGACCAGAGAGT	CCCGATCCATGCAGTAACAT	50.0	50.0	56.1	57.7	1103 bp	1.0	62.9