Hotspot

rpl14

LSC · 84899-85267 · 369 bp

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Pi 0.00271

MarkerSeek score 50.40

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 1
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	GCATGAGACACAATCTATTAATCGG	CCACGGATTTCTTTATCTCTGGA	40.0	43.5	58.2	58.0	557 bp	1.0	40.1
rpl14_p2	GCATGAGACACAATCTATTAATCGG	ACCACGGATTTCTTTATCTCTGG	40.0	43.5	58.2	58.2	558 bp	1.0	40.1
rpl14_p3	TGCATGAGACACAATCTATTAATCG	CCACGGATTTCTTTATCTCTGGA	36.0	43.5	57.6	58.0	558 bp	1.0	40.1
rpl14_p4	GCATGAGACACAATCTATTAATCGG	CCACGGATTTCTTTATCTCTGG	40.0	45.5	58.2	56.6	557 bp	1.0	40.1
rpl14_p5	TGCATGAGACACAATCTATTAATCG	ACCACGGATTTCTTTATCTCTGG	36.0	43.5	57.6	58.2	559 bp	1.0	40.1