Hotspot

petD

LSC · 80489-81836 · 1348 bp

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Pi 0.000742

MarkerSeek score 59.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1348
variable_sites: 1
pi: 0.000742
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGGCATGTTATTTGGATACTTCT	ATCTCCGACACGATCCGTAT	34.8	50.0	55.7	58.1	1557 bp	1.0	40.1
petD_p2	AGGCATGTTATTTGGATACTTCT	AATCTCCGACACGATCCGTA	34.8	50.0	55.7	58.3	1558 bp	1.0	40.1
petD_p3	AGGCATGTTATTTGGATACTTCT	TAATCTCCGACACGATCCGT	34.8	50.0	55.7	58.3	1559 bp	1.0	40.1
petD_p4	AAGGCATGTTATTTGGATACTTCT	ATCTCCGACACGATCCGTAT	33.3	50.0	56.4	58.1	1558 bp	1.0	40.1
petD_p5	AGGCATGTTATTTGGATACTTCT	TCTCCGACACGATCCGTATA	34.8	50.0	55.7	57.5	1556 bp	1.0	40.1