Hotspot

infA

LSC · 83924-84157 · 234 bp

Back to Claderia

Pi 0.00427

MarkerSeek score 48.50

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 234
variable_sites: 1
pi: 0.00427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	TCCCTACGAAAAGGATGTTT	GGTACCCTAATTTTCTCTCGAACT	40.0	41.7	54.3	58.0	369 bp	1.0	40.2
infA_p2	ATCCCTACGAAAAGGATGTT	GGTACCCTAATTTTCTCTCGAACT	40.0	41.7	54.0	58.0	370 bp	1.0	40.2
infA_p3	TCCCTACGAAAAGGATGTTT	GGTACCCTAATTTTCTCTCGAAC	40.0	43.5	54.3	56.7	369 bp	1.0	40.2
infA_p4	ATCCCTACGAAAAGGATGTTT	GGTACCCTAATTTTCTCTCGAACT	38.1	41.7	54.9	58.0	370 bp	1.0	40.2
infA_p5	TCCCTACGAAAAGGATGTTT	GGTACCCTAATTTTCTCTCGA	40.0	42.9	54.3	54.5	369 bp	1.0	40.2