Hotspot

atpI

LSC · 15137-15880 · 744 bp

Back to Claderia

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	CGCATTGCTTGAATTGGGAT	AGTTCAATTTCTGTTAGAGGACA	45.0	34.8	57.4	55.5	820 bp	1.0	40.1
atpI_p2	CGCATTGCTTGAATTGGGAT	GAAGTTCAATTTCTGTTAGAGGACA	45.0	36.0	57.4	57.3	822 bp	1.0	40.1
atpI_p3	CGCATTGCTTGAATTGGGAT	TGAAGTTCAATTTCTGTTAGAGGAC	45.0	36.0	57.4	57.3	823 bp	1.0	40.1
atpI_p4	CGCATTGCTTGAATTGGGAT	AAGTTCAATTTCTGTTAGAGGACA	45.0	33.3	57.4	56.2	821 bp	1.0	40.1
atpI_p5	CGCATTGCTTGAATTGGGAT	AGTTCAATTTCTGTTAGAGGACAA	45.0	33.3	57.4	56.2	820 bp	1.0	40.1