Hotspot

atpA

LSC · 10836-12350 · 1515 bp

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Pi 0

MarkerSeek score 30.80

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ACGATCCATTTACATTTATGTTTG	TTCCCTTTCCTTCCGAAAAA	29.2	40.0	54.3	55.1	1581 bp	1.0	40.0
atpA_p2	ACGATCCATTTACATTTATGTTTG	TTTCCCTTTCCTTCCGAAAA	29.2	40.0	54.3	55.1	1582 bp	1.0	40.0
atpA_p3	ACGATCCATTTACATTTATGTTTG	TTTTCCCTTTCCTTCCGAAA	29.2	40.0	54.3	55.1	1583 bp	1.0	40.0
atpA_p4	ACGATCCATTTACATTTATGTTTG	TTTTTCCCTTTCCTTCCGAA	29.2	40.0	54.3	55.1	1584 bp	1.0	40.0
atpA_p5	ACGATCCATTTACATTTATGTTTG	TTTCCCTTTCCTTCCGAAAAA	29.2	38.1	54.3	55.9	1582 bp	1.0	40.0