Hotspot

rpl23-rpl2

IRa · 158513-158536 · 24 bp

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Pi 0.0417

MarkerSeek score 76.50

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 24
variable_sites: 1
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	CGCTTCAACCGGGTTATTCT	CACTTGACTGTCTACGGCTC	50.0	55.0	58.3	58.0	134 bp	1.0	78.3
rpl23-rpl2_p2	CGCTTCAACCGGGTTATTCT	CCACAACGATGCTGTCCATA	50.0	50.0	58.3	58.0	181 bp	1.0	78.1
rpl23-rpl2_p3	CGCTTCAACCGGGTTATTCT	GACTGTCTACGGCTCCATTG	50.0	55.0	58.3	58.1	129 bp	1.0	77.9
rpl23-rpl2_p4	ATCATTACGCTTCAACCGGG	CACTTGACTGTCTACGGCTC	50.0	55.0	58.3	58.0	141 bp	1.0	77.7
rpl23-rpl2_p5	ATCATTACGCTTCAACCGGG	CCACAACGATGCTGTCCATA	50.0	50.0	58.3	58.0	188 bp	1.0	77.6