Hotspot

ycf1

SSC · 128981-134473 · 5493 bp

Pi 0.00663

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 187
pi: 0.00663
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTACAGACAAACGACGGGG	AGGGGTGCTGTTTCTTTCAG	50.0	50.0	58.1	58.0	212-218 bp	1.0	86.8
ycf1_p2	TTTTTATCCAAAACGCGGCC	AGGGGTGCTGTTTCTTTCAG	45.0	50.0	57.9	58.0	533-536 bp	1.0	85.5
ycf1_p3	TTTTTATCCAAAACGCGGCC	GAGGGGTGCTGTTTCTTTCA	45.0	50.0	57.9	58.0	534-537 bp	1.0	85.4
ycf1_p4	TTTTTATCCAAAACGCGGCC	GGGGTGCTGTTTCTTTCAGA	45.0	50.0	57.9	58.0	532-535 bp	1.0	85.4
ycf1_p5	TTTTTATCCAAAACGCGGCC	GGAAGCCTGGGTTGATATGG	45.0	55.0	57.9	58.0	1158-1161 bp	1.0	83.4