Hotspot

ccsA-ndhD

SSC · 120265-120602 · 338 bp

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Pi 0.0202
MarkerSeek score 48.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
338
variable_sites
26
pi
0.0202
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.93
markerseek_score
48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATCCACGGCTCTTCTAGTCA CGGACAAGGCTGAAGCTATT 50.0 50.0 57.9 58.0 848-882 bp 1.0 85.3
ccsA-ndhD_p2 TCCACGGCTCTTCTAGTCAT CGGACAAGGCTGAAGCTATT 50.0 50.0 57.9 58.0 847-881 bp 1.0 85.3
ccsA-ndhD_p3 TGTGGCTTCTATGGGCTTTC CGGACAAGGCTGAAGCTATT 50.0 50.0 57.9 58.0 455-489 bp 0.628571 72.8
ccsA-ndhD_p4 TGTGGCTTCTATGGGCTTTC TCTGGACCACGGGAGTTATT 50.0 50.0 57.9 58.1 546-580 bp 0.628571 71.4
ccsA-ndhD_p5 TGTGGCTTCTATGGGCTTTC TTCTGGACCACGGGAGTTAT 50.0 50.0 57.9 58.1 547-581 bp 0.628571 71.4