Hotspot

rpl32-trnL_uag

SSC · 118015-119128 · 1114 bp

Back to Citrus

Pi 0.013
MarkerSeek score 56.80
Primer available yes
Label rpl32-trnL_uag

Feature Metadata

label_name
rpl32-trnL_uag
feature_type
igs
region
SSC
length_bp
1114
variable_sites
75
pi
0.013
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_uag_p1 AGCGAAATCCCTTGCTACTG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 859-1233 bp 1.0 89.6
rpl32-trnL_uag_p2 AGCGAAATCCCTTGCTACTG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 860-1234 bp 1.0 89.6
rpl32-trnL_uag_p3 AGCGAAATCCCTTGCTACTG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 869-1243 bp 1.0 88.6
rpl32-trnL_uag_p4 GCGTTGAAGGCTTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 879-1253 bp 1.0 88.4
rpl32-trnL_uag_p5 GCGTTGAAGGCTTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 880-1254 bp 1.0 88.4