Hotspot

ndhF-rpl32

SSC · 116831-117855 · 1025 bp

Back to Citrus

Pi 0.00926
MarkerSeek score 54.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1025
variable_sites
60
pi
0.00926
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTTTGTTGCGGTCGGAAAAA TCCAAAGATTTTTACGAATACG 40.0 31.8 57.4 52.9 1159-1203 bp 1.0 48.1
ndhF-rpl32_p2 TTTTTGTTGCGGTCGGAAAA TCCAAAGATTTTTACGAATACG 40.0 31.8 57.4 52.9 1160-1204 bp 1.0 48.1
ndhF-rpl32_p3 TTTTGTTGCGGTCGGAAAAA TTCCAAAGATTTTTACGAATACG 40.0 30.4 57.4 53.8 1160-1204 bp 1.0 48.1
ndhF-rpl32_p4 TTTTTGTTGCGGTCGGAAAA TTCCAAAGATTTTTACGAATACG 40.0 30.4 57.4 53.8 1161-1205 bp 1.0 48.1
ndhF-rpl32_p5 TTTTTGTTGCGGTCGGAAAAA TCCAAAGATTTTTACGAATACG 38.1 31.8 58.0 52.9 1160-1204 bp 1.0 48.1