Hotspot

petL-petG

LSC · 69738-69931 · 194 bp

Back to Citrus

Pi 0.0178
MarkerSeek score 56.30
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
194
variable_sites
13
pi
0.0178
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 TCCTTTAGCTCTGTCATGCC CAACTGATCACCACGTCTGT 50.0 50.0 57.3 57.8 406-418 bp 1.0 77.1
petL-petG_p2 CCTTTAGCTCTGTCATGCCT CAACTGATCACCACGTCTGT 50.0 50.0 57.3 57.8 405-417 bp 1.0 77.0
petL-petG_p3 TTCCTTTAGCTCTGTCATGCC CAACTGATCACCACGTCTGT 47.6 50.0 58.0 57.8 407-419 bp 1.0 74.6
petL-petG_p4 TCCTTTAGCTCTGTCATGCC CCAACTGATCACCACGTCTG 50.0 55.0 57.3 58.6 407-419 bp 1.0 73.8
petL-petG_p5 TCCTTTAGCTCTGTCATGCC AAGGTCCAACTGATCACCAC 50.0 50.0 57.3 57.4 412-424 bp 1.0 73.7