Hotspot

petA-psbJ

LSC · 66364-67460 · 1097 bp

Back to Citrus

Pi 0.00728
MarkerSeek score 57.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1097
variable_sites
38
pi
0.00728
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.0 58.1 1223-1245 bp 1.0 83.8
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC TCCGGATTAGGTTCGTCCTT 50.0 50.0 58.0 58.1 1224-1246 bp 1.0 83.2
petA-psbJ_p3 CATTACGTGTCCAAGGCCTT CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.2 58.1 1220-1242 bp 1.0 82.7
petA-psbJ_p4 GCATCTGTTGTTTTGGCACA CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 1188-1210 bp 1.0 82.5
petA-psbJ_p5 TTGGCATCTGTTGTTTTGGC CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 1191-1213 bp 1.0 82.4