Hotspot

accD-psaI

LSC · 61863-62631 · 769 bp

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Pi 0.0077
MarkerSeek score 56.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
769
variable_sites
28
pi
0.0077
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CGGCCGTTTTCCTTTGAATC TTGCCATTGCCGGAAATACT 50.0 45.0 58.0 58.2 850-871 bp 1.0 82.8
accD-psaI_p2 TTATTTCTGTTCCACGGCCG TTGCCATTGCCGGAAATACT 50.0 45.0 58.6 58.2 864-885 bp 1.0 78.4
accD-psaI_p3 CGGCCGTTTTCCTTTGAATC TGCCATTGCCGGAAATACTA 50.0 45.0 58.0 57.3 849-870 bp 1.0 78.2
accD-psaI_p4 AAAAGGTGTTCTGAGCGAGT TTGCCATTGCCGGAAATACT 45.0 45.0 57.4 58.2 883-904 bp 1.0 77.8
accD-psaI_p5 AAAGGTGTTCTGAGCGAGTT TTGCCATTGCCGGAAATACT 45.0 45.0 57.4 58.2 882-903 bp 1.0 77.8