Hotspot

ndhK-ndhC

LSC · 53168-53231 · 64 bp

Back to Citrus

Pi 0.0146
MarkerSeek score 60.10
Primer available yes
Label ndhK-ndhC

Feature Metadata

label_name
ndhK-ndhC
feature_type
igs
region
LSC
length_bp
64
variable_sites
6
pi
0.0146
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK-ndhC_p1 AGCTGGAGAGTCTTGACCAA GGTTTAGTTTATGCGTGGCG 50.0 50.0 58.3 57.8 204-219 bp 1.0 81.7
ndhK-ndhC_p2 AAGCTGGAGAGTCTTGACCA GGTTTAGTTTATGCGTGGCG 50.0 50.0 58.3 57.8 205-220 bp 1.0 81.6
ndhK-ndhC_p3 AGCTGGAGAGTCTTGACCAA GTTTATGCGTGGCGAAAAGG 50.0 50.0 58.3 58.4 198-213 bp 1.0 80.1
ndhK-ndhC_p4 AAGCTGGAGAGTCTTGACCA GTTTATGCGTGGCGAAAAGG 50.0 50.0 58.3 58.4 199-214 bp 1.0 80.0
ndhK-ndhC_p5 AGCTGGAGAGTCTTGACCAA TTTATGCGTGGCGAAAAGGA 50.0 45.0 58.3 58.5 197-212 bp 1.0 79.4