Hotspot

trnG_gcc-trnfM_cau

LSC · 39523-39709 · 187 bp

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Pi 0.0121
MarkerSeek score 57.10
Primer available yes
Label trnG_gcc-trnfM_cau

Feature Metadata

label_name
trnG_gcc-trnfM_cau
feature_type
igs
region
LSC
length_bp
187
variable_sites
12
pi
0.0121
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG_gcc-trnfM_cau_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 215-316 bp 1.0 85.9
trnG_gcc-trnfM_cau_p2 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 251-352 bp 1.0 85.6
trnG_gcc-trnfM_cau_p3 TCTTTGCCAAGGAGAAGACG CCAGTCCTTCTCTTCATCGC 50.0 55.0 57.8 58.1 272-373 bp 1.0 85.0
trnG_gcc-trnfM_cau_p4 TCTTTGCCAAGGAGAAGACG TGAGGTCACGGGTTCAAATC 50.0 50.0 57.8 57.8 213-314 bp 1.0 84.8
trnG_gcc-trnfM_cau_p5 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 235-336 bp 1.0 84.2