Hotspot

petN-psbM

LSC · 30016-31295 · 1280 bp

Back to Citrus

Pi 0.00926
MarkerSeek score 56.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1280
variable_sites
65
pi
0.00926
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT CGTTCTAGTTCCTACTGCGT 50.0 50.0 58.2 57.1 1199-1515 bp 1.0 79.3
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT AGTTCCTACTGCGTTTTTGC 50.0 45.0 58.2 56.9 1193-1509 bp 1.0 78.1
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT GTTCCTACTGCGTTTTTGCT 50.0 45.0 58.2 56.9 1192-1508 bp 1.0 78.1
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT CGTTCTAGTTCCTACTGCGT 50.0 50.0 57.6 57.1 1138-1454 bp 1.0 77.7
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT TCGTTCTAGTTCCTACTGCG 50.0 50.0 58.2 56.8 1200-1516 bp 1.0 77.0