Hotspot

trnR_ucu-atpA

LSC · 10465-10717 · 253 bp

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Pi 0.0178
MarkerSeek score 47.20
Primer available yes
Label trnR_ucu-atpA

Feature Metadata

label_name
trnR_ucu-atpA
feature_type
igs
region
LSC
length_bp
253
variable_sites
21
pi
0.0178
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.91
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR_ucu-atpA_p1 AGCCTTCCAAGCTAACGATG CTCTCACGGTGGAAGAACAG 50.0 55.0 58.0 57.9 769-806 bp 1.0 86.6
trnR_ucu-atpA_p2 AGCCTTCCAAGCTAACGATG CGAAGCTTATCCCGGAGATG 50.0 55.0 58.0 57.9 1200-1237 bp 1.0 84.4
trnR_ucu-atpA_p3 AGCCTTCCAAGCTAACGATG ATCCCTCCAAACAAGCACAG 50.0 50.0 58.0 58.1 1264-1301 bp 1.0 84.0
trnR_ucu-atpA_p4 AGCCTTCCAAGCTAACGATG GATCCCTCCAAACAAGCACA 50.0 50.0 58.0 58.1 1265-1302 bp 1.0 84.0
trnR_ucu-atpA_p5 AGCCTTCCAAGCTAACGATG TGATCCCTCCAAACAAGCAC 50.0 50.0 58.0 58.1 1266-1303 bp 1.0 83.9