Hotspot

trnH_gug-psbA

LSC · 109-540 · 432 bp

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Pi 0.0108
MarkerSeek score 52.40
Primer available yes
Label trnH_gug-psbA

Feature Metadata

label_name
trnH_gug-psbA
feature_type
igs
region
LSC
length_bp
432
variable_sites
35
pi
0.0108
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH_gug-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 438-519 bp 0.942857 85.7
trnH_gug-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 452-533 bp 0.942857 85.5
trnH_gug-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 451-532 bp 0.942857 85.5
trnH_gug-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 453-534 bp 0.942857 85.5
trnH_gug-psbA_p5 CGCATGGTGGATTCACAATC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.5 57.9 467-548 bp 0.942857 82.7