Hotspot

psaI-ycf4

LSC · 61926-62359 · 434 bp

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Pi 0.0192

MarkerSeek score 60.20

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 16
pi: 0.0192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	ACGTTCTGATCGCCAATTCA	45.0	45.0	58.0	57.9	503-516 bp	1.0	83.6
psaI-ycf4_p2	CACCACCACCTTACCCTCTA	ACGTTCTGATCGCCAATTCA	55.0	45.0	58.1	57.9	548-561 bp	1.0	83.0
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	ACGTTCTGATCGCCAATTCA	45.0	45.0	57.9	57.9	497-510 bp	1.0	83.0
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	564-577 bp	1.0	82.7
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	567-580 bp	1.0	82.6