Hotspot

trnM-CAT-atpE

LSC · 54662-54825 · 164 bp

Back to Citrullus

Pi 0.0285

MarkerSeek score 72.90

Primer available yes

Label trnM-CAT-atpE

Feature Metadata

label_name: trnM-CAT-atpE
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 9
pi: 0.0285
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAT-atpE_p1	GATGTGATGTGATAGGGCCC	CTCTCAGACGAGCTAGGACA	55.0	55.0	57.8	58.0	336-347 bp	1.0	80.6
trnM-CAT-atpE_p2	GATGTGATGTGATAGGGCCC	CGAGCTAGGACACGAGTAGA	55.0	55.0	57.8	58.1	328-339 bp	1.0	80.4
trnM-CAT-atpE_p3	GATGTGATGTGATAGGGCCC	GAGGCTATCAATGGCGTACC	55.0	55.0	57.8	58.2	310-321 bp	1.0	79.4
trnM-CAT-atpE_p4	GATGTGATGTGATAGGGCCC	TCTCAGACGAGCTAGGACAC	55.0	55.0	57.8	58.3	335-346 bp	1.0	78.8
trnM-CAT-atpE_p5	GATGTGATGTGATAGGGCCC	CTCAGGGCAAGAGACAAACA	55.0	50.0	57.8	57.7	372-383 bp	1.0	78.5