Hotspot

psbZ-trnG-GCC

LSC · 38040-38434 · 395 bp

Back to Citrullus

Pi 0.00802

MarkerSeek score 61.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 6
pi: 0.00802
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	560-687 bp	1.0	82.0
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	GAATCGAACCCGCATCTTCT	55.0	50.0	58.0	58.1	561-688 bp	1.0	82.0
psbZ-trnG-GCC_p3	CTTCTCCTGATGGTTGGTCG	CATTATCCTCATCCTGGGCG	55.0	55.0	58.0	57.9	589-716 bp	1.0	81.5
psbZ-trnG-GCC_p4	GATGGTTGGTCGGGTAACAA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	552-679 bp	1.0	80.6
psbZ-trnG-GCC_p5	GATGGTTGGTCGGGTAACAA	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	553-680 bp	1.0	80.6