Hotspot

trnT-GGT-psbD

LSC · 33670-34757 · 1088 bp

Back to Citrullus

Pi 0.00888

MarkerSeek score 60.60

Primer available yes

Label trnT-GGT-psbD

Feature Metadata

label_name: trnT-GGT-psbD
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 18
pi: 0.00888
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGT-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1193-1244 bp	1.0	81.9
trnT-GGT-psbD_p2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1207-1258 bp	1.0	81.8
trnT-GGT-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1196-1247 bp	1.0	81.8
trnT-GGT-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1210-1261 bp	1.0	81.6
trnT-GGT-psbD_p5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	1209-1260 bp	1.0	81.4