Hotspot

atpH-atpI

LSC · 14872-15814 · 943 bp

Back to Citrullus

Pi 0.00848

MarkerSeek score 56.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 16
pi: 0.00848
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.0	57.9	1028-1029 bp	1.0	79.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	999-1000 bp	1.0	79.6
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.2	57.9	1029-1030 bp	1.0	78.7
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1000-1001 bp	1.0	78.5
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	GCTGCAGCTTATATAGGCGA	45.0	50.0	58.0	57.6	1016-1017 bp	1.0	78.1