Hotspot

trnR-TCT-atpA

LSC · 10831-11387 · 557 bp

Back to Citrullus

Pi 0.0178

MarkerSeek score 67.10

Primer available yes

Label trnR-TCT-atpA

Feature Metadata

label_name: trnR-TCT-atpA
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 18
pi: 0.0178
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-atpA_p1	TGGAATGAAAAGCGTCCATTG	TCCACGAAGACATTCACCAA	42.9	45.0	57.4	57.1	710-733 bp	1.0	65.7
trnR-TCT-atpA_p2	TGGAATGAAAAGCGTCCATTG	TTCCACGAAGACATTCACCA	42.9	45.0	57.4	57.1	711-734 bp	1.0	65.7
trnR-TCT-atpA_p3	GGAATGAAAAGCGTCCATTGT	TCCACGAAGACATTCACCAA	42.9	45.0	57.4	57.1	709-732 bp	1.0	65.7
trnR-TCT-atpA_p4	GGAATGAAAAGCGTCCATTGT	TTCCACGAAGACATTCACCA	42.9	45.0	57.4	57.1	710-733 bp	1.0	65.7
trnR-TCT-atpA_p5	TGGAATGAAAAGCGTCCATTG	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	684-707 bp	1.0	65.0