Hotspot

ycf1

SSC · 128055-133544 · 5490 bp

Back to Citropsis

Pi 0.00597

MarkerSeek score 29.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5490
variable_sites: 49
pi: 0.00597
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACGAAATGCATGTTCCCCT	CGAAGAAGCGCGGTCTTTAT	45.0	50.0	58.1	58.7	245-254 bp	1.0	75.5
ycf1_p2	ACGAAATGCATGTTCCCCTT	CGAAGAAGCGCGGTCTTTAT	45.0	50.0	58.1	58.7	244-253 bp	1.0	75.5
ycf1_p3	ACGTTTGGCTTTTCGTGAAC	CGAAGAAGCGCGGTCTTTAT	45.0	50.0	57.8	58.7	262-271 bp	1.0	74.6
ycf1_p4	GAACGAAATGCATGTTCCCC	CGAAGAAGCGCGGTCTTTAT	50.0	50.0	57.7	58.7	246-255 bp	1.0	73.9
ycf1_p5	GCAACCACTAAACGTTTGGC	CGAAGAAGCGCGGTCTTTAT	50.0	50.0	58.5	58.7	273-282 bp	1.0	71.8