Hotspot

rpl20

LSC · 71596-71949 · 354 bp

Back to Citropsis

Pi 0.00942

MarkerSeek score 34.50

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 354
variable_sites: 5
pi: 0.00942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	TGAAAGAAGTGGGTTGACCG	GAGCCGAAGAGTGGTAACTG	50.0	55.0	58.0	58.0	1015-1024 bp	1.0	81.3
rpl20_p2	TGAAAGAAGTGGGTTGACCG	TGGCTCCGGTGTATAGAGAG	50.0	55.0	58.0	58.0	959-968 bp	1.0	81.3
rpl20_p3	TGAAAGAAGTGGGTTGACCG	CAGCTACCCCACCAATCTTC	50.0	55.0	58.0	58.0	1106-1115 bp	1.0	80.9
rpl20_p4	TTTGTGTCACCCTTCCAAGG	GAGCCGAAGAGTGGTAACTG	50.0	55.0	57.9	58.0	1444-1453 bp	1.0	80.8
rpl20_p5	TTTGTGTCACCCTTCCAAGG	TGGCTCCGGTGTATAGAGAG	50.0	55.0	57.9	58.0	1388-1397 bp	1.0	80.7