Hotspot

ndhF

SSC · 113674-115911 · 2238 bp

Back to Citropsis

Pi 0.00688

MarkerSeek score 35.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 23
pi: 0.00688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TATCCGCCGAAGAAGATCCT	AAGAACCGGCGAATCAGAAA	50.0	45.0	58.0	57.8	2522-2525 bp	1.0	79.7
ndhF_p2	TATCCGCCGAAGAAGATCCT	TGGGGAACGATAGGAGTCAG	50.0	55.0	58.0	58.2	2865-2869 bp	1.0	79.2
ndhF_p3	CAGGAACAAGAGGTATCCGC	AAGAACCGGCGAATCAGAAA	55.0	45.0	58.1	57.8	2535-2538 bp	1.0	79.2
ndhF_p4	TATCCGCCGAAGAAGATCCT	TTGGGGAACGATAGGAGTCA	50.0	50.0	58.0	57.8	2866-2870 bp	1.0	79.1
ndhF_p5	GTATCCGCCGAAGAAGATCC	AAGAACCGGCGAATCAGAAA	55.0	45.0	57.9	57.8	2523-2526 bp	1.0	79.1