Hotspot

rpl32-trnL-UAG

SSC · 117124-118203 · 1080 bp

Back to Citropsis

Pi 0.0144

MarkerSeek score 45.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1080
variable_sites: 22
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCGAAATCCCTTGCTACTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1181-1188 bp	1.0	85.4
rpl32-trnL-UAG_p2	AGCGAAATCCCTTGCTACTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1182-1189 bp	1.0	85.4
rpl32-trnL-UAG_p3	AGGTATCTAAGGGAGTCGCC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.0	58.0	1585-1597 bp	1.0	84.8
rpl32-trnL-UAG_p4	AGGTATCTAAGGGAGTCGCC	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.0	58.0	1586-1598 bp	1.0	84.8
rpl32-trnL-UAG_p5	GTCGCCAATAACTCCGGAAT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1571-1583 bp	1.0	84.4