Hotspot

trnW-CCA-trnP-UGG

LSC · 69383-69553 · 171 bp

Back to Citropsis

Pi 0.0195

MarkerSeek score 52.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 5
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	AAGACGACAAGAGCGGAATC	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	302-307 bp	1.0	80.5
trnW-CCA-trnP-UGG_p2	AAGACGACAAGAGCGGAATC	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	303-308 bp	1.0	80.5
trnW-CCA-trnP-UGG_p3	AAGACGACAAGAGCGGAATC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	291-296 bp	1.0	80.5
trnW-CCA-trnP-UGG_p4	AAGACGACAAGAGCGGAATC	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	289-294 bp	1.0	80.5
trnW-CCA-trnP-UGG_p5	AAGACGACAAGAGCGGAATC	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	292-297 bp	1.0	80.5