Hotspot

psbZ-trnG-GCC

LSC · 38582-39128 · 547 bp

Back to Citropsis

Pi 0.00975

MarkerSeek score 51.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 8
pi: 0.00975
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	695-741 bp	1.0	76.6
psbZ-trnG-GCC_p2	TTTGCTTCTCCGGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	705-751 bp	1.0	75.8
psbZ-trnG-GCC_p3	ATTTGCTTCTCCGGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	706-752 bp	1.0	75.4
psbZ-trnG-GCC_p4	GGATGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	694-740 bp	1.0	73.5
psbZ-trnG-GCC_p5	CGTTGTATTTGCTTCTCCGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	712-758 bp	1.0	73.1