Hotspot

trnT-GGU-psbD

LSC · 34014-35264 · 1251 bp

Back to Citropsis

Pi 0.00815
MarkerSeek score 50.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1251
variable_sites
15
pi
0.00815
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1396-1414 bp 1.0 81.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1399-1417 bp 1.0 81.4
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1398-1416 bp 1.0 81.4
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1401-1419 bp 1.0 81.3
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG AAAAACGAATCGGTCCCTCC 55.0 50.0 58.1 58.2 1380-1398 bp 1.0 80.3