Hotspot

petN-psbM

LSC · 29863-31138 · 1276 bp

Back to Citropsis

Pi 0.0106

MarkerSeek score 40.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 20
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AAGTACGCGACTCTTGACAC	TTGTACAAGTAAGGCGGTCG	50.0	50.0	58.0	57.9	2467-2476 bp	1.0	81.2
petN-psbM_p2	GTACGCGACTCTTGACACTT	TTGTACAAGTAAGGCGGTCG	50.0	50.0	58.0	57.9	2465-2474 bp	1.0	81.2
petN-psbM_p3	ACATTTTCCCTTTCGCTCGT	TTGTACAAGTAAGGCGGTCG	45.0	50.0	58.1	57.9	1764-1779 bp	1.0	81.0
petN-psbM_p4	AAGTACGCGACTCTTGACAC	GCGGTTAGGTGGTTGTACAA	50.0	50.0	58.0	58.1	2479-2488 bp	1.0	80.8
petN-psbM_p5	GTACGCGACTCTTGACACTT	GCGGTTAGGTGGTTGTACAA	50.0	50.0	58.0	58.1	2477-2486 bp	1.0	80.8