Hotspot

rpoB-trnC-GCA

LSC · 27775-28977 · 1203 bp

Back to Citropsis

Pi 0.0061

MarkerSeek score 50.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 11
pi: 0.0061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCGCATTTCCATCTCC	ATCGTTTGTTGATCAGGCGA	50.0	45.0	58.0	57.9	1314-1355 bp	1.0	79.8
rpoB-trnC-GCA_p2	ATTCCCGCATTTCCATCTCC	ACCAGCCAAGCAGAATAAGG	50.0	50.0	58.0	57.9	1347-1388 bp	1.0	79.5
rpoB-trnC-GCA_p3	ATTCCCGCATTTCCATCTCC	GTTTGTTGATCAGGCGACAC	50.0	50.0	58.0	57.7	1311-1352 bp	1.0	78.0
rpoB-trnC-GCA_p4	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1273-1314 bp	1.0	77.7
rpoB-trnC-GCA_p5	ATTCCCGCATTTCCATCTCC	CAGCCAAGCAGAATAAGGGA	50.0	50.0	58.0	57.6	1345-1386 bp	1.0	77.1