Hotspot

trnQ-UUG-psbK

LSC · 7515-7866 · 352 bp

Back to Citropsis

Pi 0.0189

MarkerSeek score 38.40

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 10
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	AAGAGGGTTGCTCAAAAGGG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	646 bp	1.0	81.5
trnQ-UUG-psbK_p2	GAGGGTTGCTCAAAAGGGAA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	644 bp	1.0	81.5
trnQ-UUG-psbK_p3	AAGAGGGTTGCTCAAAAGGG	GCCTCGGGTAATTTAGCGAA	50.0	50.0	58.0	58.1	556 bp	0.333333	52.9
trnQ-UUG-psbK_p4	GAGGGTTGCTCAAAAGGGAA	GCCTCGGGTAATTTAGCGAA	50.0	50.0	58.0	58.1	554 bp	0.333333	52.8
trnQ-UUG-psbK_p5	CGGGGAACACAAATGCATTC	GCCTCGGGTAATTTAGCGAA	50.0	50.0	58.0	58.1	700 bp	0.333333	52.8