Hotspot

trnR-UCU

LSC · 9722-9793 · 72 bp

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Pi 0.0725

MarkerSeek score 74.20

Primer available yes

Label trnR-UCU

Feature Metadata

label_name: trnR-UCU
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 5
pi: 0.0725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	ACGAATAAACCGCAGTTCCA	50.0	45.0	58.0	57.8	425 bp	1.0	82.1
trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	ACGAATAAACCGCAGTTCCA	55.0	45.0	58.1	57.8	428 bp	1.0	81.9
trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	CCCCTCTCACGGTAGAAGAA	50.0	55.0	58.0	58.2	544 bp	1.0	81.5
trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	CCCCTCTCACGGTAGAAGAA	55.0	55.0	58.1	58.2	547 bp	1.0	81.2
trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	TTGGCAAGAGGTCAACGATT	50.0	45.0	58.0	57.7	593 bp	1.0	80.3