Hotspot

rrn23

IRb · 106099-108908 · 2810 bp

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Pi 0.00214

MarkerSeek score 47.60

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: IRb
length_bp: 2810
variable_sites: 6
pi: 0.00214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	CAGCACCTTAGGATGGCATA	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.1	57.9	2937 bp	1.0	71.9
rrn23_p2	CAGCACCTTAGGATGGCATA	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.1	57.9	2962 bp	1.0	71.9
rrn23_p3	TTAGCAGCACCTTAGGATGG	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.0	57.9	2941 bp	1.0	71.3
rrn23_p4	TTAGCAGCACCTTAGGATGG	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.0	57.9	2966 bp	1.0	71.3
rrn23_p5	CAGCACCTTAGGATGGCATAA	AAACTTCTGTCGCTCCATCC	47.6	50.0	57.8	57.9	2937 bp	1.0	69.7