Hotspot

rpl32-trnL-UAG

SSC · 115631-116766 · 1136 bp

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Pi 0.0176

MarkerSeek score 57.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1136
variable_sites: 18
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.1	1176-1283 bp	1.0	84.6
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCGTTAA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.0	58.1	1178-1285 bp	1.0	84.6
rpl32-trnL-UAG_p3	GGGATATTGGGCAGCGTTAA	GTTAAGAGGCCCGAAGTAGC	50.0	55.0	58.0	58.1	1695-1795 bp	1.0	84.5
rpl32-trnL-UAG_p4	GGGATATTGGGCAGCGTTAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1165-1272 bp	1.0	84.3
rpl32-trnL-UAG_p5	GGGATATTGGGCAGCGTTAA	ATTGGAGGGCGGGTACTAAT	50.0	50.0	58.0	58.2	1736-1836 bp	1.0	83.5