Hotspot

trnA-UGC-rrn23

IRb · 105950-106098 · 149 bp

Back to Cistanthe

Pi 0.0738

MarkerSeek score 69.90

Primer available yes

Label trnA-UGC-rrn23

Feature Metadata

label_name: trnA-UGC-rrn23
feature_type: igs
region: IRb
length_bp: 149
variable_sites: 11
pi: 0.0738
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-rrn23_p1	GGCTGTCAAGAACGTAGAGG	ATTCGGGAATCTCCGGATCT	55.0	50.0	58.0	58.0	969-1003 bp	1.0	80.9
trnA-UGC-rrn23_p2	GGGCTGTCAAGAACGTAGAG	ATTCGGGAATCTCCGGATCT	55.0	50.0	58.0	58.0	970-1004 bp	1.0	80.9
trnA-UGC-rrn23_p3	GGCTGTCAAGAACGTAGAGG	AGTGATGCTTTCGGCTACTG	55.0	50.0	58.0	58.0	1215-1249 bp	1.0	80.8
trnA-UGC-rrn23_p4	GGGCTGTCAAGAACGTAGAG	AGTGATGCTTTCGGCTACTG	55.0	50.0	58.0	58.0	1216-1250 bp	1.0	80.8
trnA-UGC-rrn23_p5	GGCTGTCAAGAACGTAGAGG	ATGTTTCAGTTCGCCAGGTT	55.0	45.0	58.0	58.0	1036-1070 bp	1.0	80.8