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rps8-rpl14

LSC · 82742-82905 · 164 bp

Back to Cistanthe

Pi 0.0183

MarkerSeek score 63.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 3
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.7	58.0	268-271 bp	1.0	78.6
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	57.7	58.1	266-269 bp	1.0	77.8
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	AACTCGAGTTTTTGGTGCGA	50.0	45.0	57.7	58.3	267-270 bp	1.0	76.1
rps8-rpl14_p4	CAGCAATAGTGTCCCTACCC	GAACTCGAGTTTTTGGTGCG	55.0	50.0	57.4	58.0	269-272 bp	1.0	75.8
rps8-rpl14_p5	AGCAATAGTGTCCCTACCCA	GGAACTCGAGTTTTTGGTGC	50.0	50.0	57.7	57.6	269-272 bp	1.0	75.6