Hotspot

psaA-ycf3

LSC · 42511-43477 · 967 bp

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Pi 0.0166

MarkerSeek score 68.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 16
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	1150-1153 bp	1.0	80.2
psaA-ycf3_p2	GTTCTTGAAAAATGGCCCGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1169-1172 bp	1.0	80.2
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	CGATAACGCTTACTCCTGGG	50.0	55.0	58.1	57.9	1123-1126 bp	1.0	79.5
psaA-ycf3_p4	GTTCTTGAAAAATGGCCCGG	CGATAACGCTTACTCCTGGG	50.0	55.0	57.9	57.9	1142-1145 bp	1.0	79.5
psaA-ycf3_p5	GTCTGGCCCATTCCTCAAAA	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	1152-1155 bp	1.0	79.4