Hotspot

trnC-GCA-petN

LSC · 27520-28127 · 608 bp

Back to Cistanthe

Pi 0.0164

MarkerSeek score 68.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 10
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTTCCCCAGTTCAAATCCG	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.1	58.3	664 bp	1.0	78.4
trnC-GCA-petN_p2	TGTTCCCCAGTTCAAATCCG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	726 bp	1.0	77.5
trnC-GCA-petN_p3	ACTGCAAATCCTTGTTCCCC	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	676 bp	1.0	76.2
trnC-GCA-petN_p4	GCAAATCCTTGTTCCCCAGT	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	673 bp	1.0	76.2
trnC-GCA-petN_p5	TGCAAATCCTTGTTCCCCAG	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	674 bp	1.0	76.2