Hotspot

rpoC1-rpoB

LSC · 23200-23225 · 26 bp

Back to Cistanthe

Pi 0.0385

MarkerSeek score 64.30

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGTTGAGGAGAAACTGATCCA	ACGATCTTTGGCTCTGGAAC	42.9	50.0	56.6	57.9	158 bp	1.0	60.3
rpoC1-rpoB_p2	AGGAGAAACTGATCCAATTCGT	ACGATCTTTGGCTCTGGAAC	40.9	50.0	57.4	57.9	153 bp	1.0	59.2
rpoC1-rpoB_p3	TGTTGAGGAGAAACTGATCCA	TCGACTCCTCGTTCGAGAAT	42.9	50.0	56.6	58.3	179 bp	1.0	58.9
rpoC1-rpoB_p4	TGTTGAGGAGAAACTGATCCA	CTTTTCGACTCCTCGTTCGA	42.9	50.0	56.6	57.7	183 bp	1.0	58.4
rpoC1-rpoB_p5	TGTTGAGGAGAAACTGATCCA	TTTTCGACTCCTCGTTCGAG	42.9	50.0	56.6	57.7	182 bp	1.0	58.4