Hotspot

trnG-UCC-trnR-UCU

LSC · 9665-9721 · 57 bp

Back to Cistanthe

Pi 0.0566
MarkerSeek score 71.40
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
57
variable_sites
3
pi
0.0566
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG ACGAATAAACCGCAGTTCCA 50.0 45.0 58.0 57.8 425 bp 1.0 82.1
trnG-UCC-trnR-UCU_p2 AGCCTTCCAAGCTAACGATG CCCCTCTCACGGTAGAAGAA 50.0 55.0 58.0 58.2 544 bp 1.0 81.5
trnG-UCC-trnR-UCU_p3 CCTAGCCTTCCAAGCTAACG CCCCTCTCACGGTAGAAGAA 55.0 55.0 58.1 58.2 547 bp 1.0 81.2
trnG-UCC-trnR-UCU_p4 GGGTCTCCCGGTTTGATTAC CCCCTCTCACGGTAGAAGAA 55.0 55.0 58.0 58.2 1228 bp 1.0 79.9
trnG-UCC-trnR-UCU_p5 GGGTCTCCCGGTTTGATTAC ACGAATAAACCGCAGTTCCA 55.0 45.0 58.0 57.8 1109 bp 1.0 79.9