Hotspot

trnS-GCU-trnG-UCC

LSC · 8068-8878 · 811 bp

Back to Cistanthe

Pi 0.0174

MarkerSeek score 57.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 14
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	934-935 bp	1.0	70.8
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.2	56.8	934-935 bp	1.0	70.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AACGAATCGCACTTTTACCAC	50.0	42.9	58.2	57.5	935-936 bp	1.0	68.4
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	CGAATCGCACTTTTACCACT	50.0	45.0	58.2	56.5	933-934 bp	1.0	68.4
trnS-GCU-trnG-UCC_p5	CTTTCGTCCACTCAGCCATC	ACGAATCGCACTTTTACCACT	55.0	42.9	58.6	58.2	870-871 bp	1.0	67.0